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BCA vs Bradford: How Far Off Each Assay Reads 13 Proteins Against a BSA Standard
A protein-by-protein bias table for BCA, Micro BCA, Modified Lowry, Bradford and Bradford Plus, built from the vendor's own response ratios, plus an A280 column computed from UniProt.
Runcell Blog14 min readPrimer Dimer Check Tutorial: Testing ΔG Rules on the CDC qPCR Primers
We ran the CDC SARS-CoV-2 N1, N2, N3 and RNase P primers and probes through two dimer models and Primer3. A consecutive-pair checker at common ΔG limits misses the N3 set that CDC withdrew.
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Computed ε280, molecular weight and Abs 0.1% for 11 common lab proteins from UniProt mature chains. Shows how far the '1 A280 = 1 mg/mL' shortcut is off (0.17× to 2.65×), what pasting the precursor sequence costs, and why nucleic-acid contamination skews proteins with a low Abs 0.1% the most.
Runcell Blog15 min readJupyter to PDF: A Measured Fidelity Benchmark of nbconvert's Three Export Paths
Nine probe notebooks, three export paths, twenty-seven conversion attempts, and a count of what actually survives into each PDF. The LaTeX route keeps every column of a wide DataFrame and drops four of eight Unicode strings; the browser routes do the reverse, and clip a long source line as well.
Runcell Blog31 min readModuleNotFoundError in Jupyter: A Reproduced Kernel Failure Matrix
The package imports in your terminal and fails in the notebook. Four virtual environments, nine notebook runs, and the one line of jupyter_client source that decides which Python your kernel actually runs — with the audit script that answers it for your machine.
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Learn how to deploy OpenClaw, a self-hosted AI Gateway that connects to WhatsApp, Telegram, and Discord. Step-by-step setup with deployment options included.
Runcell Blog5 min readRDKit Python Tutorial for Chemists (With Examples)
Step-by-step RDKit tutorial for chemists. Install RDKit, draw molecules, calculate properties, and run similarity searches in Python — no coding experience required.
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Runcell Blog6 min readDESeq2 in Python: Step-by-Step PyDESeq2 Tutorial
Run DESeq2 differential expression analysis entirely in Python with PyDESeq2. Covers count matrices, normalization, statistical testing, LFC shrinkage, and visualization.
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What Jupyter's .ipynb_checkpoints folders are, whether it is safe to delete them, and why adding them to .gitignore does not untrack the ones you already committed - with a throwaway repository showing exactly what each untracking command does to your index.
Runcell Blog8 min readHow to Install Jupyter Notebook on macOS
Install Jupyter Notebook on Mac with a venv, Conda, Homebrew, or JupyterLab Desktop — including the real "externally-managed-environment" error a global pip install hits on Homebrew Python, and the versions a clean venv install gets today.
Runcell Blog8 min read
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