3 stories
A protein-by-protein bias table for BCA, Micro BCA, Modified Lowry, Bradford and Bradford Plus, built from the vendor's own response ratios, plus an A280 column computed from UniProt.
Computed ε280, molecular weight and Abs 0.1% for 11 common lab proteins from UniProt mature chains. Shows how far the '1 A280 = 1 mg/mL' shortcut is off (0.17× to 2.65×), what pasting the precursor sequence costs, and why nucleic-acid contamination skews proteins with a low Abs 0.1% the most.
Why ng/µL → nM calculators disagree by 7%, measured on real NCBI sequences with Biopython. Covers free acid vs sodium salt, the 650/660/330 rules of thumb, 5' phosphates, and a full pUC19 digest-to-ligation workflow.
Showing 3 of 3 stories
You're all caught up.
Get the top AI news and tools straight to your inbox every week.
Weekly · AI news
Join 15,000+ AI enthusiasts
